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Protein Purification

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Protein purification involves a sequence of procedures that aim to extract one or a few proteins from a complex mixture, which may comprise cells, tissues, or entire organisms. The purification of proteins is critical for understanding the structure, function, and interactions of the protein of interest.


AKTA avant

BFF has extensive knowledge in protein purification and experience with untagged proteins required for clinical studies.





       Our services include: 

                  - Size Exclusion           

                  - Ion Exchange

                 - Affinity (Ni-NTA, Protein G)

                 - Hydrophobic Interactions

                 - Reverse Phase HPLC

                 - ÄKTA Avant for scouting and method optimization 

                 - Scale-up protein production (up to 25L packed resin columns)

                 - Protocol development